By A. Garrido Frenich
Publication via Frenich, A. Garrido, Plaza-bolanos, P., Aguilera-luiz, M. M., Martinez-vidal, J. L.
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Extra resources for Veterinary drugs and growth-promoting agent analyses
An extensive list of CID fragments for sulphonamides is already published in literature [6,92]. 8. Quinolones Reversed-phase LC is the first choice for the separation of quinolones; GC is not applied for this purpose due to the high polarity of these VDs. Certain ampholytic compounds, such as enrofloxacine and its TP, ciprofloxacin, can show peak tailing problems because of the presence of silanol groups and metal traces that interact with them. In consequence, the use of end-capped or high purity silica columns and the optimization of pH and ion-strength conditions are strongly recommended .
Despite MS detection is preferred for the analysis of nitroimidazoles, UV detection has also been applied [110,111], for instance, as screening tool prior to LC–MS/MS with APCI or ESI techniques for confirmation purposes [29,106]. Figure 4. 8, 20 mM NH4Ac). ]. Chromatographic Techniques 31 Nitrofurans Because of these compounds are rapidly metabolized, the analysis of nitrofuran residues is limited to the determination of their main metabolites. Due to the high polarity of the metabolites, separation on reversed-phase columns is unfavorable and the retention is poor.
Helix pomatia juice (a species of snail, also known as Roman snail) is widely used to achieve this goal due to its content in β-glucuronidase and arylsulphatase. However, the presence of oxidoreductase enzyme activity can provoke the reaction of the steroid 3-ol group to a 3-oxo group . g. sulphates, glucoronides, glycosides and glutathiones) due to the high polarity of these analytes; the combination of ESI and QqQ has been described as the optimum choice [9,120]. Moreover, LC–MS can be used for trenbolone-like steroids (thermolabile compounds), stanozolol (strong adsorption in GC) and progestagens (strong non-polarity) .