By F. Yvonne Schulman

Veterinarian's consultant to Maximizing Biopsy effects helps veterinary practitioners in acquiring high quality biopsy samples, maximizing the implications and minimizing the cost.

  • Details the biopsy method step through step
  • Includes a submission record to guarantee thoroughness
  • Helps clinicians keep away from error while amassing biopsy samples
  • Ensures fine quality samples for better effects and diminished costs
  • Provides sensible, clinically orientated information

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Extra info for Veterinarian’s Guide to Maximizing Biopsy Results

Sample text

Iii. Handle tissue carefully to minimize squeeze artifact. Fenestrated forceps are reported to cause less squeeze artifact than non-fenestrated instruments (Golden 1993). Once the specimen is collected, gently tease it from the forceps with a needle. The specimens can then be placed on a formalin-soaked cassette sponge with the deep surface against the sponge for orientation and immediately placed in formalin (Willard et al. 2001) or directly placed in formalin. Do not put fresh specimens in cassettes with dry cassette sponges.

Figure 13 Photomicrograph of a good-quality endoscopic biopsy specimen of small intestine. The sample includes superficial (villi) and deep (crypts) mucosa with no significant squeeze artifact. Figure 14 Photomicrograph of poor-quality endoscopic biopsy specimen of small intestine. Only villi were collected. These specimens can be lost during tissue processing, provide excessively small amounts of tissue for evaluation and preclude examination of the deep mucosa. Figure 15 Drawing of how endoscopic biopsy specimens cannot adequately sample a mural lesion.

7 Spleen Biopsy of the spleen is usually prompted by splenomegaly or splenic masses. Splenic masses often contain extensive areas of congestion, hemorrhage, necrosis, inflammation, and/or fibrosis that can mask underlying disease, making adequate sampling paramount (Figures 16–19). Figure 16 A splenic mass with areas of congestion, necrosis, and hemorrhage. Thin slices (less than 1 cm) including all grossly different-appearing areas allow for proper fixation and adequate sampling to identify the lesion (hemangiosarcoma in this case).

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