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Physiological free hormone concentrations are - 11 1 1 estimated to be approximately 1 χ 1 0 M for T 3 and 5 χ Ι Ο M for T 4 . These values, which were obtained by equilibrium dialysis (Sterling and Brenner, 1966), should be considered as approximations of free hormone levels but may not likely represent exact values present in vivo. Although thyroid hormone-free serum could probably be utilized from a variety of different animal species, serum obtained from a thyroidectomized calf was chosen for several reasons.

V. VI. I. H. SAMUELS Introduction Cell Culture Considerations Effect of Thyroid Hormones in Cultured G H , Cells A. Regulation of Growth Hormone Production by Thyroid Hormone B. Identification of Cellular Receptors for the Thyroid Hormones C. The Relationship between Thyroid Hormone Nuclear Binding and the Biological Response in G H X Cells 1. Comparison of the Growth Hormone Response to T 3 Nuclear Binding 2. Relationship of Thyroid Hormone Nuclear Receptor Levels by Triiodothyronine Quantitation of Growth Hormone m R N A and Regulation of Glucocorticoid Action by Thyroid Hormone in G H !

Sei. A. 72, 325. Surks, M. , and Defesi, Ch. R. (1977). Endocrinology 104, 946. Surks, M. , and Oppenheimer, J. H. (1977). J. Clin. Invest. 60, 555. Surks, M. , Dillmann, W. , and Oppenheimer, J. H. (1973). J. Biol. Chem. 248, 7066. Surks, M. , Koerner, D. , and Oppenheimer, J. H. (1975). J. Clin. Invest. 55, 50. Tarentino, A. , Richert, D. , and Westerfeld, W. W. (1966). Biochim. Biophys. Acta Tata, J. R. (1975). Nature {London) 257, 18. Tata, J. , and Widnell, C. C. (1966). Biochem. J. 98, 604.

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