By James H. Clark

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This is insufficient time for labeling of cellular phospholipid pools to iso­ topic equilibrium. Evaluation of GnRH-stimulated phosphoinositide me­ tabolism by nonequilibrium labeling with 32P has the disadvantage that the monitored effects seen under these conditions are far removed from the likely proximate event, a phospholipase C-type reaction. Therefore, the effects seen are likely to be indicative of altered rates of turnover of phospholipid pools and do not necessarily reflect changes in the mass of these pools.

Tsai-Morris, C. , and Dufau, M. L. (1985). Endocrinology 117, 2544-2546. , and Csernus, V. (1983). Andrologia 15, 398-403. , and Bardin, C. W. (1986). Endocrinology 115,1645-1647. , and Bloom, F. (1977). Science 197, 1367-1369. Gunsalus, G. , Musto, N. , and Bardin, C. W. (1980). In "Testicular Development, Structure and Function" (A. Steinberger and E. ), pp. 291-297. Raven, New York. Gunsalus, G. , Vogel, D. , Musto, N. , and Bardin, C. W. (1984). In "Sexual Differentiation: Basic and Clinical Aspects" (M.

Indeed, several lines of evidence indicate that ligands 34 P. MICHAEL CONN ET AL. with the ability to bring receptors together are able to act as agonists in some systems. , 1978). Studies using monovalent and divalent Fab forms of the insulin receptor antibodies (prepared by enzymatic digestion) indicate that both fragments are able to bind to the receptor, but that the agonist activity is observed only with divalent antibodies. Antibodies directed against the TSH receptor also activate thyroxine production in the thyroid (review see, Blecher and Bar, 1981).

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