By P. Michael Conn

As a result of value of morphology and mobile verbal exchange specific to the functioning and interdependence of neural platforms, the various advances within the neurosciences depend upon vintage in addition to new microscopy concepts.

A handy bench-top format**Methods offered for simple model to new systems**Comprehensive protocols incorporated for**Autoradiography: size of hormone receptors, high-resolution concepts, double labeling and mixed techniques**Statistical and computational tools: video imaging, 3-dimensional reconstructions, technique of structural quantitation, independent sampling tools, size of research of neuronal connections, staining, and person axon arbors**Tracing neuronal connections: tracer, toxin, and dye methods**Staining concepts: fluoro-gold, SITS, and silver impregnation**Freezing suggestions: freeze fracture and cryoprotection**Combined and high-resolution strategies: double-label tracer suggestions, incident gentle polarization, gentle and electron microscopic strategies

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50 I AUTORADIOGRAPHY 17. F. M. Leslie and C. A. " Liss, New York, 1988. 18. W. E. Stumpf, AEC Symp. Ser. 13, 633 (1988). 19. I. Leav, F. B. Merk, P. Ofner, G. Goodrich, P. W. L. Kwan, B. M. Stein, M. Sar, and W. E. Stumpf, Am. J. Pathol. 93, 69 (1978). 20. W. E. Stumpf and M. Sar, in "Receptors and Mechanism of Action of Steroid Hormones: Modern Pharmacology-Toxicology" (J. ), Vol. 8, p. 41. Dekker, New York, 1976. 21. M. Geusken, M. J. Burglen, and J. Uriel, Virchows Arch. B 24, 67 (1977). [3] High-Resolution Autoradiographic Imaging of Brain Activity Patterns with Radiolabeled 2-Deoxyglucose and Glucose Gary E.

H. Feder, Endocrinology (Baltimore) 106, 1061 (1980). 45. A. Maggi, I. Zucchi, and J. Perez, Pharmacol. Res. Commun. 17, 283 (1985). 46. M. Y. McGinnis, L. C. Krey, N. J. MacLusky, and B. S. McEwen, Neuroendocrinology 33, 158 (1981). 47. R. H. Hochberg, Science 205, 1138 (1979). 48. J. E. Zielinski, H. Yabuki, S. L. Pahuja, J. M. Larner, and R. B. Hochberg, Endocrinology (Baltimore) 119, 130 (1986). 49. R. B. Hochberg, R. M. Hoyte, and W. Rosner, Endocrinology (Baltimore) 117, 2550 (1985). 50. J.

Stumpf and M. Sar, in "Receptors and Mechanism of Action of Steroid Hormones: Modern Pharmacology-Toxicology" (J. ), Vol. 8, p. 41. Dekker, New York, 1976. 21. M. Geusken, M. J. Burglen, and J. Uriel, Virchows Arch. B 24, 67 (1977). [3] High-Resolution Autoradiographic Imaging of Brain Activity Patterns with Radiolabeled 2-Deoxyglucose and Glucose Gary E. Duncan and Walter E. Stumpf Development of the autoradiographic 2-deoxy[14C]glucose [14C]2-DG) technique by Sokoloff et al. (1) was a major advance of the investigation of regional brain glucose metabolism.

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