By Murray P. Deutscher, Jiren Zhang (auth.), John E. G. McCarthy, Mick F. Tuite (eds.)
The final ten years have witnessed a notable bring up in our expertise of the significance of occasions next to transcriptional initiation by way of the law and keep an eye on of gene expression. particularly, the improvement of recombinant DNA recommendations that started within the Seventies supplied robust new instruments with which to check the molecular foundation of keep watch over and rules in any respect degrees. The ensuing investigations published a range of post-transcriptional mechanisms in either prokaryotes and eukaryotes. Scientists engaged on translation, mRNA balance, transcriptional (anti)termination or different features of gene expression will frequently have met at expert conferences for his or her personal study quarter. despite the fact that, purely hardly ever do staff in numerous parts of post-transcriptional regulate/ legislation be able to fulfill less than one roof. We consequently inspiration it was once time to compile prime representatives of many of the correct components in a small workshop meant to motivate interplay around the ordinary borders of analysis, either when it comes to the procedures studied, and with appreciate to the evolutionary department prokaryotes/eukaryotes. Given the breadth of subject matters lined and the constraints in measurement imposed via the NATO workshop layout, it used to be a very tricky job to settle on the contributors. notwithstanding, we looked this primary try out as an test on a small scale, meant to discover the probabilities of a gathering of this type. Judging by way of the reaction of the members in the course of and after the workshop, the hassle have been worthwhile.
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Extra resources for Post-Transcriptional Control of Gene Expression
5 kb pufBA mRNA segment were similar under high or low oxygen growth conditions. 5 kb pufmRNA segments. Investigations of the stability of mRNA encoded by constructs containing deletions or insertions in the puf operon suggest the rate of endonucleolytic cleavage within the RC-coding region is affected by oxygen (Klug, submitted for publication). 5 min ,1+-~-r~-'--~~~-r~~ o 20 40 60 80 100 time (min) Figure 6: Decay of purmRNA under high (20%, - - -) or low (1-2%, - - -) oxygen determined from Northrn blot experiments.
Transcription was addi tion of IPTG. were then E. col i was transformed with pUCEI0 and induced from the lac promoter by the The RNA was extracted and the transcripts characterized by primer extension with an oligonucleotide complementary to +1 to +20 of gene 32. To verify that the -71 site processing dependent, we extracted RNA from wild type is and RNase E RNase E ts mutant strains at both 30·C and 43·C. The results of primer extension analysis of these RNAs are shown in figure i. A band corresponding to a 5' end at -71 (arrow) is detected both at 30·C and 43·C in the wild-type strain (rne+; lanes 1,3).
Von Gabain, D. Georgellis, U. Lundberg, Melin and O. Resnekov Dept. of Bacteriology Karolinska Institute 10401 Stockholm Sweden O. Melefors, L. Abstract The expression of many genes is adjusted in response to changes in bacterial growth rate or growth stage. One mechanism which affects gene expression is the control of mRNA stability. coli ompA gene (encoding the outer membrane protein A) The is an example where the stability of the mRNA changes in response to changes in the bacterial growth rate.