By Z. Li, R. L. Jarret, J. W. Demski (auth.), Professor Dr. Y. P. S. Bajaj (eds.)
Twenty-seven chapters care for the regeneration of crops from protoplasts and genetic transformation in a variety of species of Arachis, Buplerum, Capsella, Dendrobium, Dianthus, Diospyros, Fagopyrum, Festuca, Gentiana, Glycyrrhiza, Gossypium, Hemerocallis, Levisticum, Lonicera, Musa, Physalis, Platanus, Prunus, Saposhikovia, Solanum, Spinacia, Trititrigia, Tulipa, together with culmination comparable to apricot, banana, cranberry, pepino, peach, and plum. those stories replicate the far-reaching implications of protoplast know-how in genetic engineering of crops. they're of detailed curiosity to analyze scientists, academics and complex scholars within the fields of plant tissue tradition, molecular biology, genetic engineering, plant breeding and basic biotechnology.
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Additional info for Plant Protoplasts and Genetic Engineering VI
This promoting effect is ascribed to the lowering of osmotic pressure and growth regulator level (Jia et al. 1986; Praveen et al. 1986); but the cause of this effect (or "mechanism") seems different in different stages of culture, as shown in this experiment. If the culture was supplemented on the 7th and 17th day by either culture solution without glucose and growth regulator or by solution of original composition in the proportion of 1:10 (V) (added solution: culture), there was no obviouulifference in the promoting effect between them.
In: Institute of Materia Medica, Chinese Academy of Medical Sciences (eds) Flora of Chinese medicinal plants. People's Hygienic Press, Beijing, Vol 2 481-500 Wang Ii-mei, Chen Hui-min (1991) Plant regeneration from protoplasts of Peucedanum praeruptorum Dunn. Acta Bot Sin 33: 261-266 Wang Tie-zeng, Wu Zhu-jun, Chen Zhong-ming (1982) Fangfeng (Saposhnikovia divaricata) In: Institute of Materia Medica, Chinese Academy of Medical Sciences (eds) Flora of Chinese Medical Plants. ) Medic. -C. c. A.
In B. scorzonerifolium, modified MS medium (see protocol) was preferable to modified N6 (see protocol) and KM8p (Kao and Michayluk 1975); 30--50 Regeneration of Bupleurum scorzoneriJolium and Saposhnikovia divaricata 17 Fig. 1(1-13). Bupleurum scorzoneriJolium 1 Freshly isolated protoplasts; bar=50 ~m . 2,3 First asymmetrical division; bar=80 ~m 4 Three- and four-celled colonies; bar=70 ~m 5 A small protocolony with polarity of cell arrangement after 7 days of culture; bar=50 ~m. 6,7 Multicellular globular proembryo after about 2 months of culture; bar=50 ~m .