By Hille E., Tamarkin J. D.

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G. in the meristem or localized after Genetic dissection of plant stress responses 23 pathogen induction, can suggest the possible phenotype to screen for. The entrapment inserts allow for the selection of inserts in specific classes of genes, based on their expression pattern. Subsequent production of double or multiple mutants in a pathway or between partially redundant genes, as indicated by the expression pattern, might finally reveal mutant phenotypes. g. with a TATA box, situated near the border of the insert.

1998. Floral dip: a simplified method for Agrobacteriummediated transformation of Arabidopsis thaliana. Plant J. 16,735-743. C. 1999. Mutations affecting induction of glycolytic and fermentative genes during germination and environmental stresses in Arabidopsis. Plant Physiol. 119, 599-608. Das, L. and Martienssen, R. 1995. Site-selected transposon mutagenesis at the hcfl06locus in maize. Plant Cell7, 287-294. , Kyozuka, J. and Shimamoto, K. 1999. Ac as a tool for the functional genomics of rice.

1991; Topping and Lindsey, 1995), suggesting that T-DNA inserted preferentially in transcriptionally active regions. Large TDNA collections have been produced in Arabidopsis that can be used for forward as well as reverse genetics strategies (Table 2). , 1997). From a screen of 2500 lines two tagged lines displayed low-temperature enhanced expression that was also ABA responsive. For transposons similar strategies were employed for the Ac-Ds transposons engineered as promoter, enhancer or gene/exon trap systems.

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