By George O. Poinar Jr., Gerard M. Thomas (auth.)

After the booklet of the Diagnostic guide for the id of Insect Pathogens, the authors acquired many queries asking why that they had no longer incorporated the bigger metazoan parasites in addition to the microbial types. An exam of the literature indicated that pictorial publications to the identity of nematodes and the immature phases of insect parasites have been unavailable. as a result we made up our minds to rewrite the sections conceal­ ing insect pathogens and mix those with new sections on ento­ mogenous nematodes and the immature levels of insect parasites. the result's the current laboratory advisor, that is precise in overlaying all kinds of biotic brokers that are chanced on within bugs and reason them harm or ailment. integrated as parasites are bugs and nematodes. one of the pathogens incorporated are viruses, rickettsias, micro organism, fungi, and protozoans. Emphasis is put on id with an try and use the main simply recognizable characters. Use of a undeniable variety of technical phrases is unavoidable, and causes of those are available in such a lot organic dictionaries or the word list of invertebrate pathology ready by means of Steinhaus and Martignoni (1970).

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VIRUSES C M E FIGURE 9. TEM section which illustrates the paracrystalline nature of the capsule granulin. A cross section shows the virus core (C), capsid (M), and envelope (E). ) x 40,000. -. • 10 :, . # ~ ••• 00-• -' • • FIGURE 10. Sectioned adipose tissue of Choristoneura fumiferana infected with entomopoxvirus. The fat cells lose their granules and numerous spheroids (arrows) appear. (Courtesy of E. } x2000. VIRUSES FIGURE 11. A squash mount of the scarabaeid grub Cyc/ocepha/a immaculata infected with an entomopoxvirus.

X 140,000. VIRUSES C M E FIGURE 9. TEM section which illustrates the paracrystalline nature of the capsule granulin. A cross section shows the virus core (C), capsid (M), and envelope (E). ) x 40,000. -. • 10 :, . # ~ ••• 00-• -' • • FIGURE 10. Sectioned adipose tissue of Choristoneura fumiferana infected with entomopoxvirus. The fat cells lose their granules and numerous spheroids (arrows) appear. (Courtesy of E. } x2000. VIRUSES FIGURE 11. A squash mount of the scarabaeid grub Cyc/ocepha/a immaculata infected with an entomopoxvirus.

43 44 VIRUSES FIGURE 26. A nonoccluded nuclear virus (V) with a polydisperse DNA genome originating from the calyx of the braconid wasp Phanerotoma flavitestacea invading tissue of the host, Amye/ois transitella (Lepidoptera). ) X66,OOO. VIRUSES FIGURE 27. Negatively stained preparation of chronic bee paralysis virus, illustrating the anisometric shape (mainly ellipsoidal). (Courtesy of L. ) x200,OOO. 45 46 VIRUSES FIGURE 28. Negatively stained preparations of the filamentous virus of the honeybee.

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