By Stuart McLaughlin (auth.), G. C. Papageorgiou, J. Barber, S. Papa (eds.)

The professional Committee on Biomaterials and Biotechnology for the ecu and the North American quarter used to be based by way of the overall meeting of UNESCO at its twenty first consultation, in 1981. The Committee includes a Coordinating workforce and 4 operating teams, outlined within the following medical parts: team I Proteins: resource, constitution and serve as. staff II Nucl~ic acids: the hereditary fabrics. workforce III Im~une fabrics and mechanisms. Membranes and delivery in biosystems. team IV In fulfilment of 1 of the targets of the Committee, that have been followed via the overall meeting of UNESCO in 1981, particularly the intensification of the alternate of medical info on biomaterials and biotechnology, operating staff IV geared up a world workshop on Ion Interactions in strength shipping structures, which used to be convened in Athens, Greece, from eight to twelve April, 1985. medical papers awarded at that workshop make up the chapters offered during this quantity. the current quantity focusses on normal and synthetic membranes which are fascinated with power transduction. a number of chapters are dedicated to membranes and membrane parts that convert and make the most of mild, akin to the thylakoid membrane of oxygenic photosynthetic organisms (eukaryotic and prokaryotic), the chromatophore membrane of nonoxygenic photosynthetic micro organism and the red membrane of the halophilic micro organism. different platforms tested comprise the mitochondrial membranes and their adenine nucleotide provider, the plasma membrane of animal cells, and lipid bilayer vesicles, both reconstituted or no longer, with enzymes.

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Thus, screening of the potential is approached from both positive and negative values. The extrapolated fluorescence intensity indicate'the intensity at zero surface potential (F o ). Since, at low concentration of dye the fluorescence is proportional to the binding constant and the binding constant is a function of the surface potential (see below), than 'YS=591og (FIFo) (mV). l ~30 • II.! } -12 C ..... 6 ..... ,. ;:, " - Q.. ,. q " '0. DD "- - -- - - - - - ~ - - - ~'- I o~ '. 0 10· FIGURE 1: Salt dependence of ANS fluorescence and the calculated surface potential (Robertson and Rottenberg ref.

28. induced thylakoid stacking and the chlorophyll fluorescence changes associated with changes in spillover of energy from photosystem II to photosystem I. FEBS Lett. , Scoufflaire, C. and Lannoye, C. (1980) The relationship between thylakoid stacking and salt induced chlorophyll fluorescence changes. Biochim. Biophys. , Larsson, C. and Akerlund, H-E. (1982) Phase partition - A method for purification and analysis of cell organelles and membrane vesicles. Meth. Biochem. Anal. S. and Barber, J.

Tion, this value is about 14 millivolts. In our particular prepara- However, the magnitude of this change is likely to vary with method of preparation. ~ Department of Energy under contract No. DE-AC0376SF00098. REFERENCES (1) S. McLaughlin, Electrostatic Potentials at Membrane-Solution Interfaces, Current Topics Membrane Trans. 9: 71-126 (1977) (2) G. Schaefer and G. Rowohl-Quisthoudt, Influence of Surface Potentials on the Mitochondrial H+ Pump and on Lipid Phase Transitions, J. Bioenergetics 8: 73-81 (1976).

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