By S. Mohan Jain (ed.), S. K. Sopory (ed.), R. E. Veilleux (ed.)

Since the start of agricultural creation, there was a continual attempt to develop extra and higher caliber nutrition to feed ever expanding popula­ tions. either more advantageous cultural practices and more desirable crop vegetation have allowed us to divert extra human assets to non-agricultural actions whereas nonetheless expanding agricultural creation. Malthusian inhabitants predictions proceed to alarm agricultural researchers, in particular plant breeders, to hunt new applied sciences that might proceed to permit us to provide extra and higher nutrients through fewer humans on much less land. either development of current cultivars and improvement of recent high-yielding cultivars are universal ambitions for breeders of all plants. In vitro haploid creation is likely one of the new applied sciences that exhibit nice promise towards the aim of accelerating crop yields via making comparable germplasm on hand for plenty of vegetation that used to be used to enforce one of many maximum plant breeding luck tales of this century, i. e. , the improvement of hybrid maize through crosses of inbred strains. one of many major purposes of anther tradition has been to supply diploid homozygous natural traces in one iteration, hence saving many generations of backcrossing to arrive homozygosity by way of conventional potential or in plants the place self-pollination isn't attainable. simply because doubled haploids are comparable to inbred traces, their price has been preferred by way of plant breeders for many years. the hunt for common haploids and techniques to urge them has been ongoing because the starting of the 20 th century.

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Commun. 1: 28-31. S. (1981) The preliminary studies on in vitro culture of unfertilized ovaries of rice. Acta Bot. Sin. 24: 33-38. Lai, M. & L. Chen (1984) Induction of rice plantlets from anther culture. Bot. Bull. Acad. Sin. 17: 18-24. M. C. Chen (1987) Nuclear fusion in cultured microspores of barley. Plant Cell Rep. 6: 191-193. Lee, J. C. Chen (1982) Genetic and histological evidence for microspore origin of antherderived plants of rice. Taiwania 27: 86-92. Lenka, N. M. Reddy (1994) Role of media, plant growth regulators in callusing and plant regeneration from anthers of Indica rice.

T. Abe & Y. Futsuhara (1987). Int. Rice Genet. News!. 4: 109-110. Kameya, T. & K. Hinata (1970) Induction of haploid plants from pollen grains of Brassica. Jpn. J. Breed. 20: 82-87. H. J. Zapata (1990) One step rice plantlet development through anther culture. Indian J. Plant Physiol. 33: 119-124. , M. M. Reddy (1989) Plant regeneration from haploid callus of Indica rice. Proc. Indian Nat!. Sci. Acad. 55B: 193-202. T. Y. S. Kim (1991) Anther culture efficiency in different rice genotypes under different cold pretreatment durations and culture temperatures.

Sativa (Rout & Sarma, 1991). S. Gosal et al. , 1989). Callus formation and plant regeneration could be enhanced by 1 mg l- 1 kin and 2 mg l- 1 NAA, but 2 mg l- 1 kin resulted in more albino plants. Kavi Kishore et al. (1989) regenerated plants at a higher frequency on R 2 medium containing 2 mg l- 1 each kin and IAA or LS medium with 1 mg l- 1 IAA and 4 mg l- 1 kin. Similarly, Draz et al. (1991) observed plant regeneration on media with 5 mg l- 1 kin and 1 mg l- 1 IAA or 2 mg l- 1 kin and 1 mg l- 1 IAA.

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