This handbook, to be released in volumes, presents a condensed evaluation of the analytical research of eighty chinese language natural medications that are most often in use.

Thin layer chromatographic-, excessive strain liquid chromatographic- and gasoline chromatographic-fingerprint analytical innovations permit the detection of all major low-molecular parts of a plant drug or even unmarried materials may be visualized. Analytical effects thereof are proven in different colour figures.

The caliber facts of the research meets the traditional of the eu Drug Regulatory Authority. in addition, this quantity supplies a close description of the analytical tools used for numerous medications. Bioactive parts, pharmacological and organic actions of numerous unmarried natural medicinal drugs in addition to their healing functions are mentioned.

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Additional resources for Chromatographic Fingerprint Analysis of Herbal Medicines: Thin-Layer and High Performance Liquid Chromatography of Chinese Drugs, Volume 1 (2nd Edition)

Sample text

Saikosaponin b1 – b4 are artefacts of saponins a and d, which arise during extraction of the plant juices in acid medium by splitting off the 13ß, 28-epoxy group(3, 8), monoacetylsaikosaponins and acidic saponins, which are derived from oleanolic acid(7). – sapogenins: the saikogenins E, F and G are recognized as being genuine, while the saikogenins A, B, C and D are regarded as artefacts of the latter (4, 9). *) – neutral phytosterols such as α-spinasterol and stigmasterol. – fatty acids such as palmitic, oleic, linoleic and stearic acid(5, 7), polyhydroxysterols.

Scorzonerifolium can be distinguished from B. chinense by that way. B. chinense may be differentiated from the Japanese species only by the two characteristic violet-blue spots at Rƒ 0,15. HPLC ¿ngerprint analysis 1) Sample preparation: 2 ml extract (10 g drug/10 ml MeOH) are concentrated to dryness at 40 – 60°C under vacuum, and the residue dissolved in 1 ml distilled H2O. The aqueous suspension is then treated 1 – 2 minutes in a ultrasonic bath to dissolve any residue adhering to the Àask. , followed by 10 ml distilled water.

Hypotensive effect: verticine and verticinone in high doses; at low doses the contrary effect becomes evident(5). – sedative effect: verticine and verticinone antagonize the stimulatory effect of caffeine and potentiate the sedative effect of chlorpromazine in mice(5). In humans, preparations of Fritillariae bulbus exhibit antitussive and expectorant effects(3). Fig. 1: Formulae of the main compounds TLC ¿ngerprint analysis: 1) Extraction: 10 g coarsely-ground drug are treated for 1 hr with 50 ml 0,1 N sulphuric acid in an ultrasonic bath under occasional stirring.

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