By Maria Lindgren, Ülo Langel (auth.), Ülo Langel (eds.)

In fresh years, a brand new knowing of cell-penetrating peptides has emerged, aiding researchers to extend past a few long-held dogmas. In Cell-Penetrating Peptides: equipment and Protocols, specialist researchers discover the most recent details on cell-penetrating peptides (CPPs), delivering perception into an important and modern components of CPP study. Chapters tackle the historic history of CPP reviews, supply an summary of the growing to be box of analysis, examine tools for trying out CPP mechanisms, current a precis of tools that try to use homes of CPPs to check biochemical intracellular mechanisms of interplay and sign transduction, and contain new rules for turning CPP-based ideas into medicines. Composed within the hugely profitable equipment in Molecular Biology™ sequence layout, every one bankruptcy features a short creation, step by step tools, a listing of priceless fabrics, and a Notes part which stocks pointers on troubleshooting and keeping off recognized pitfalls. cutting edge and present, Cell-Penetrating Peptides: equipment and Protocols is an important advisor that permits researchers to review intracellular mechanisms in new methods, and to advertise the long run improvement of novel drugs.

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Beck-Sickinger, A. , and Merkle, H. P. (2005) Decoding the entry of two novel cell-penetrating peptides in HeLa cells: lipid raft-mediated endocytosis and endosomal escape. Biochemistry 44, 72–81. 60. , and Sugiura, Y. (2001) Stearylated arginine-rich peptides: a new class of transfection systems. Bioconjug Chem 12, 1005–11. 61. , Kogan, M. , and Giralt, E. (2006) Replacement of a proline with silaproline causes a 20-fold increase in the cellular uptake of a pro-rich peptide. J Am Chem Soc 128, 8479–83.

24. , and Langel, Ü. (1998) Galanin-based peptides, galparan and transportan, with receptor-dependent and independent activities. Ann N Y Acad Sci 863, 450–3. 25. , and Langel, Ü. (1998) Cell penetration by transportan. FASEB J 12, 67–77. 26. , Hao, J. , Xu, X. , and Langel, Ü. (1998) Cell penetrating PNA constructs regulate galanin receptor levels and modify pain transmission in vivo. Nat Biotechnol 16, 857–61. 27. , and Langel, Ü. (2006) Cellpenetrating peptides as vectors for peptide, protein and oligonucleotide delivery.

76. Morris, M. , and Divita, G. (2000) Translocating peptides and proteins and their use for gene delivery. Curr Opin Biotechnol 11, 461–6. 77. , Arzumanov, A. , Moulton, H. , Ivanova, G. , Iversen, P. , Gait, M. , and Lebleu, B. (2007) Cellpenetrating-peptide-based delivery of oligonucleotides: an overview. Biochem Soc Trans 35, 775–9. 78. , and Divita, G. (2008) Delivery of proteins and nucleic acids using a non-covalent peptide-based strategy. Adv Drug Deliv Rev 60, 537–47. 79. Turner, J. , Ivanova, G.

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