By Vipin K Agarwal; American Chemical Society. Division of Agricultural and Food Chemistry

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S. Patent 4,238,521. 6. E. K. Ruey, 1988. " J. Assoc. , P304-316. 7. E. Charm, E. Zomer, and R. Salter. S. " J. of Food Protection, Vol 51, No. 12, P920-924, 1988. 8. G. Guest, 1988. A. A. CVM Advisory Committee Meeting, April 12, Rockville, MD. 53 9. B. Ingersoll, 1989. " Wall street Journal, Friday, December 29. 10. H. Thomas, 1990. A. CVM April 3, Washington. 11. M. J. L. M. W. M. Karpman, 1990. A. O. - Report to the Chairman, Human Resources and Intergov. Relations Subcommittee. S. O. Box 6015 - Gaithersburg, MD 20877.

Raw Milk ITankl I Screen go Chann II Tes! Sample Nag 775,695 1608 I f>g!! ;i)lCllnel ...... ·· . .. .. .. .. .. .. ; ••• ;••• , ... ;••• j ••• ;... ; ••• ;••• ; ... ·, . .. .. . .. , ... , ............ lalioo prgcedYra Using ColD and Rgptor Assay Sarnpli Nag 761 1396 I f>g!! 980 HPLC-Rgplorgram Sample o o 14. I Charm nTell (BlllBo) positive for chlortetracycline at 25 ppb and tetracycline at 5ppb UVof o~~~~~~~~~~~~~~~~Sta~s • • 7 • ft • • v • M • • Altltnllon TIme (mlnJ B • Figure 3.

A comparison of the CFT with these other tests shows it is more sensitive for some drugs than the methods in use as well as the USDA FAST test (34) which is still under development. This 47 TABLE 6. D. (ppb) CHARM FARM TEST 150 20 1 150 25 3,200 12 NA 1,000 80 TABLE 7. A. Tolerance Levels in Egg* DRUG FAMILY ANTIMICROBIAL DRUG TOLERANCE (ppb) Aminoglycosides Streptomycin Beta-lactams Macrolides PeniciliinG Erythromycin Tylosin Sulfa Drugs Sulfadimethoxine Tetracyclines Sulfamethazine Sulfaquinoxaline Chlortetracycline Hygromycin B * CFR Reference 48 0 0 25 200 100 100 100 0 0 is particularly true for sulfa drugs and tetracyclines (see Table 8).

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